Molecular Cloning has served as the foundation of technical

expertise in labs worldwide for 30 years. No other manual has been

so popular, or so influential. Molecular Cloning, Fourth Edition,

by the celebrated founding author Joe Sambrook and new co-author,

the distinguished HHMI investigator Michael Green, preserves the

highly praised detail and clarity of previous editions and includes

specific chapters and protocols commissioned for the book from

expert practitioners at Yale, U Mass, Rockefeller University, Texas

Tech, Cold Spring Harbor Laboratory, Washington University, and

other leading institutions. The theoretical and historical

underpinnings of techniques are prominent features of the

presentation throughout, information that does much to help

trouble-shoot experimental problems.

For the fourth edition of this classic work, the content has

been entirely recast to include nucleic-acid based methods selected

as the most widely used and valuable in molecular and cellular

biology laboratories.

Core chapters from the third edition have been revised to

feature current strategies and approaches to the preparation and

cloning of nucleic acids, gene transfer, and expression analysis.

They are augmented by 12 new chapters which show how DNA, RNA, and

proteins should be prepared, evaluated, and manipulated, and how

data generation and analysis can be handled.

The new content includes methods for studying interactions

between cellular components, such as microarrays, next-generation

sequencing technologies, RNA interference, and epigenetic analysis

using DNA methylation techniques and chromatin immunoprecipitation.

To make sense of the wealth of data produced by these techniques, a

bioinformatics chapter describes the use of analytical tools for

comparing sequences of genes and proteins and identifying common

expression patterns among sets of genes.

Building on thirty years of trust, reliability, and authority,

the fourth edition of Molecular Cloning is the new gold

standard—the one indispensable molecular biology laboratory manual

and reference source.

JOSEPH SAMBROOK：

Sambrook was educated at the University of Liverpool (BSc (hons)

1962) and obtained his PhD at the Australian National University in

1966. He did postdoctoral research at the MRC Laboratory of

Molecular Biology (1966-67) and the Salk Institute for Biological

Studies (1967-69). In 1969 he was hired by James D. Watson to work

at the Cold Spring Harbor Laboratory in New York. Watson has been

reported to say this was the best hiring decision he ever made. Joe

was responsible for creating a combative creative environment at

CSHL that fomented discovery. Subsequently he worked at the

University of Texas Southwestern Medical Center (Dallas).

Sambrook is best known for his studies on DNA tumor viruses and

the molecular biology of normal and neoplastic cells. His Tumour

Virus Group at Cold Spring Harbor identified and mapped all of the

major genes of adenoviruses and SV40, determined their

transcriptional control in infected and transformed cells, and

elucidated the mechanism of integration of these viruses into the

genome of the host cell.[citation needed] He has also made

important contributions to the understanding of intracellular

traffic and protein folding and is an influential leader in the

field of the molecular genetics of human cancer.